Fig. 3: Msi1 and Pcbp2 are highly enriched in rod photoreceptors relative to other cell types (MESA compilation).
From: ASCOT identifies key regulators of neuronal subtype-specific splicing
a Analysis of splicing patterns enriched in mouse rods reveals some exons that overlap with neurons, muscles, or pancreatic α and β-cells but also exons that are rod-specific and not present in other cell types. b Similarly to other neurons, rods downregulate Ptbp1 and upregulate Ptbp2. The Ptbp2/Ptbp1 ratio gradually increases in rods over development from P2 to P28. However, we also find that cochlear hair cell sensory neurons retain high levels of Ptbp1, suggesting that perhaps not all neurons switch from using Ptbp1 to Ptbp2. To identify the factors that might mediate the splicing of rod-specific exons, we cross referenced genes with expression patterns that were enriched in rods with a list of putative RBPs and identified Msi1 and Pcbp2 as potential candidates. Note that the expression of Msi2, a paralog to Msi1, does not show the same selectively high expression in rods.
