Fig. 5: The calcium-binding site is required for Gpr126 function in vivo.
From: Structural basis for adhesion G protein-coupled receptor Gpr126 function
a Surface conservation analysis (green, variable; purple, conserved) of CUB domain. The calcium-binding site is circled in magenta. D134 and F135 are indicated by arrows. b D134 and F135 were both mutated to alanines through homologous recombination of a 150ābp ssODN containing a 5ābp mutation (red nucleotides). c Genotyping assay for the gpr126stl464 lesion. The 5ābp mutation introduces a BstUI restriction enzyme binding site. d 4 dpf wild-type larva compared to e 4 dpf gpr126stl464/stl464 larva with puffy ears (arrowheads). Scale bars (black) represent 100āĪ¼m. f 4 dpf wild-type larvae express mbp throughout the posterior lateral line nerve (PLLn, arrowhead), whereas g 4 dpf gpr126stl464/stl464 larva lack mbp expression along the PLLn (arrowhead). Scale bars (black) represent 100āĪ¼m. Asterisks indicate CNS.
