Fig. 4: A5 2D-lattice modulates the diffusivity of underlying lipids.
From: Annexin-V stabilizes membrane defects by inducing lipid phase transition
a–c Consecutive fluorescence recovery after photobleaching (FRAP) experiments on SLB membrane (DOPC/DOPS/NBD-PC 19:20:1) on glass measured in the conditions of a A5-free (SLB only), b full A5 coverage, and c A5-removal upon EDTA-addition. Two separate spectral channels with different excitation lasers were used to monitor NBD-PC and A5-Alexa 647 (mixture of A5:A5-Alexa 647 at a ratio of 2:1), respectively. The white dashed circles indicate the photobleaching area. d Time-lapse analysis of FRAP experiments within the photobleaching areas shown in a–c. The presence of the A5 2D-lattice slows down the fluorescence recovery of the underlying membrane (orange). The A5 2D-lattice is stable and shows no fluorescence recovery. e Calculated lipid diffusivity in the subsequent FRAP experiments. The formation of A5 2D-lattice decreases the mobility of underlying lipids, which was recovered by removing A5 molecules through EDTA-addition. Each data represent the mean ± s.d. (mean ± standard deviation) of n ≥ 10 FRAP areas.
