Fig. 5: TPX2-tubulin co-condensation underlies efficient branching MT nucleation.
From: Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation
a Schematic of chimera design: the endogenous N-terminal 1–480aa of TPX2 are replaced with exogenous domains containing distinct features shown in table. b Quantification of tubulin partition coefficient as a function of TPX2 concentration. For partition coefficient graphs, mean values (points) with ±1 SD as error bars from a representative experiment are plotted and a line was fit; n of condensates per condition are: IDR_NoTB—111, 80, 142, 99, 198, and 198 for 50, 100, 250, 500 nM, 1, and 2.5 μM [TPX2/TB], respectively; NoIDR_TB—198, 136, and 86 for 5, 10, and 25 μM [TPX2/TB], respectively; IDR_TB—87, 122, 153, 198, and 198 for 100, 250, 500 nM, 1, and 2.5 μM [TPX2/TB], respectively; Syn_Pos—326, 302, 100, 261, 270, and 323 for 50, 100, 250, 500 nM, 1 and 2.5 μM [TPX2/TB], respectively. See also Supplementary Figs. 6a, c, f and 7e for partition coefficients of TPX2. c Rate of MT nucleation as a function of TPX2 concentration for indicated constructs. Rates for each concentration of a given construct are normalized to the maximum rate of that construct (absolute maximum rates between all constructs are in a ±2× range). Lines of best fit shown. Data for full length (Figs. 2e and 3d) and CT_TPX2 (Fig. 4d) were previously shown. See also Supplementary Fig. 6b, d, e, g and 7f for individual rate curves and Supplementary Movies S7 and S8. Source data are provided as a Source Data file. d Summary table of results.MT nucleation efficiencies are relative to full-length TPX2.
