Fig. 4: Increased BLI signal by vaginal UP in the presence of cervical damage. | Nature Communications

Fig. 4: Increased BLI signal by vaginal UP in the presence of cervical damage.

From: Cervical epithelial damage promotes Ureaplasma parvum ascending infection, intrauterine inflammation and preterm birth induction in mice

Fig. 4

In the afternoon of D16 of gestation, mice received either 10% N-9 in PBS or PBS control via intravaginal inoculation. After 16 h, in the morning of D17 of gestation, mice received either luciferease-expressing UP in ureaplasma-selective medium (USM) or USM control via intravaginal inoculation. After 24 h, in the morning of D18, the luciferase substrate Furimazine was injected intraperitoneally to allow UP localisation. A representative image is shown a. Bioluminescence signal was quantified in a region of interest (ROI) covering each mouse’s abdomen. Bioluminescence signal coming from luciferase-expressing UP was significantly increased in mice that have been pre-treated with N-9 (n = 23) compared to PBS controls (n = 22) (b). Error bars indicate SD. Statistical significance was assessed using unpaired t test on log-transformed values of bioluminescence quantification for PBS + UP vs. N-9 + UP (*P = 0.0131) (n = 10 for PBS + USM, n = 11 for N-9 + USM). Source data are provided as a Source Data file.

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