Fig. 9: Cytokine mRNA levels in gestational tissues following vaginal UP infection. | Nature Communications

Fig. 9: Cytokine mRNA levels in gestational tissues following vaginal UP infection.

From: Cervical epithelial damage promotes Ureaplasma parvum ascending infection, intrauterine inflammation and preterm birth induction in mice

Fig. 9

In the afternoon of D16 of gestation, mice received either 10% N-9 or PBS control via intravaginal inoculation. After 16 h, in the morning of D17 of gestation, mice received either UP or USM control via intravaginal inoculation. Tissues were collected 48 h later and analysed using RT-qPCR. Mice that were infected with UP (PBS + UP, n = 6; N-9 + UP, n = 7) had an increase in the mRNA levels of TNFα (a, e, i), IL-1β (b, f, j), CXCL-1 (c, g, k) and CXCL-2 (d, h, l) compared to vehicle-treated controls (PBS + USM, n = 6). The effect was stronger at the proximal site. Error bars indicate SD. Statistical significance of the treatment effect was assessed using Dunnett’s multiple comparisons test on the DDCt values against the PBS + USM group for the proximal and distal site. Statistical significance for the site effect was assessed unpaired t test for proximal vs. distal for each treatment group (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) (n = 6 for N-9 + USM). Source data are provided as a Source Data file.

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