Fig. 3: ADAR1 activity is required to control the survival of melanocytes from E18.5.
From: ADAR1 mediated regulation of neural crest derived melanocytes and Schwann cell development
a–d Analysis of the expression of melanocytes differentiation genes (Sox10, Dct, Tyr) and Interferon-stimulated genes (ISG signature composed of Cxcl10, Isg15, Ifit1, Ifit2, Rsad2, Mx1) in the skin of controls (gray) and HtPA-Cre; Adar1fl/fl mutants (blue) at a P0, b, c E18.5 (note that we found mutant embryos of the two categories within the same litter) and d E16.5, by RT-qPCR. All data represent mean ± SEM. Statistical differences between the groups (for a n = 4 controls and n = 3–6 mutants depending on tested genes; for b n = 5 controls and n = 4 or 5 mutants depending on tested genes; for c n = 4 or 5 controls and n = 4–8 mutants depending on tested genes; for d n = 5 or 8 controls and n = 5 or 8 mutants depending on tested genes) were determined using t test (asterisks represent p values: *p < 0.05, **p < 0.01, ***p < 0.001). e YFP (green) and activated-caspase 3 (red) immunostaining on skin sections of control and HtPA-Cre; Adar1fl/fl mutant mice at E18.5 (white arrowheads indicate apoptotic cells) and quantification of caspase-3-positive cells among YFP cells (%). Data represent mean ± SEM, and statistical differences between the groups (n = 3 controls, gray and n = 6 mutants, blue) was determined using t test (asterisks represent p value: ***p < 0.001). Counterstaining with DAPI (blue) is shown to identify structures. Scale bar: 50 μm. For all panels, source data are provided as a source data file.
