Fig. 4: Phenotypes of SIRPα⁺ cells and levels of SIRPα expression.

Spleen cells harvested 24 h after vaccination with 2.5 × 10⁶ irradiated WT or CD47KO B16F0 cells were analyzed for SIRPα expression on various cell populations by flow cytometry. a Representative flow cytometry profiles showing the ratio of SIRPα⁺ cells (left), CD11b and CD11c expression gated SIRPα+ cells (middle), and levels of SIRPα expression on gated CD11b⁺CD11c⁺ DCs (open histogram), CD11b⁻CD11c⁺ DCs (gray shaded histogram) and CD11b⁺CD11c⁻ myeloid cells (black shaded histogram; right). b MFI of SIRPα expression on the indicated cell populations. **p < 0.01; ***p < 0.005 (one-way ANOVA). c Percentages of total SIRPα⁺ cells. d Percentages of SIRPα⁺CD11b⁺CD11c⁻ myeloid cells. e Percentages of total SIRPα⁺CD11c⁺, SIRPα⁺CD11c⁺CD11b⁺, and SIRPα⁺CD11c⁺CD11b⁻ DC subsets. Results are presented as mean ± SEM (n = 4 per group). *p < 0.05; **p < 0.01; ***p < 0.005; ns, not significant (unpaired t-test). Source data are provided as a Source Data file.