Fig. 2: Bach2 is required for the survival and proliferation of Treg cells.

a, b Treg cells sorted from Cd4^Cre or Bach2^fl/flCd4^Cre mice were adoptively transferred into congenically marked Foxp3 diphtheria toxin receptor transgenic mice depleted of endogenous Treg cells by injection of diphtheria toxin. a Representative flow cytometry plots indicate the frequency of donor Treg cells among the CD4⁺ compartment (left), and quantification of Treg cell numbers (right), 7 days following adoptive transfer. b Frequency of IFN-γ/TNF co-expressing cells among the splenic CD8 and conventional CD4 T cell populations, as measured by flow cytometry. c Splenic Th1 (left) and Treg (right) cell numbers from Bach2^fl/flCd4^Cre and Bach2^fl/fl control mice following infection with Plasmodium chabaudi. d Flow cytometry histograms of CellTrace Violet dilution at day 5 of culture (left) and normalized total cell numbers of Treg cells from Bach2^fl/flCd4^Cre and Bach2^fl/fl control mice at the indicated culture time points (right). e Cell-surface binding of Annexin V by Treg cells isolated from Bach2^fl/fl and Bach2^fl/flCd4^Cre mice following 24 h of culture. Flow cytometry plots and data from (b–d) are representative, data in a are pooled from two independent experiments with at least four mice per genotype. Significance tested using two-way ANOVA with the Šidák correction for multiple comparisons (d). Otherwise, statistical significance was tested using the unpaired Student’s t-test. Error bars denote mean ±S.D. a, b, d, e or ±S.E.M. c Source data are provided as a Source Data file.