Fig. 3: Bach2 restrains Treg cell suppressive function in colitis.
Bach2fl/flFoxp3Cre and Foxp3Cre control mice were administered 1.5% (w/v) dextran sodium sulfate (DSS) in drinking water for 5 days and regular drinking water for another two days. a Mouse weight over time normalized to starting weight. b Representative colons from DSS-treated Foxp3Cre and Bach2fl/flFoxp3Cre mice (left) and quantification of colon length (right). c Flow cytometry plots quantifying monocytes in the colonic lamina propria. d Histological staining of the distal colon of DSS-treated mice. Arrowhead indicates submucosal inflammatory infiltrate, solid arrow, transmural inflammatory infiltrate, dashed arrow, mucosal inflammatory infiltrate and erosion of the crypt-villus architecture. Scale bar = 200 μm. e Quantification of disease scores between genotypes. f Frequencies of Treg cells in the colonic lamina propria of DSS-treated Foxp3Cre and Bach2fl/flFoxp3Cre mice. g Flow cytometry plots showing IL-10 and KLRG1 expression by Treg cells from the lamina propria (left), and quantification of expression (right). Flow cytometry plots are representative, data are pooled from (a and b) or representative of (c–g) two independent experiments with seven control and nine conditional knockout mice. Significance tested using two-way ANOVA with Šidák correction for multiple comparisons (a). Otherwise, statistical significance was tested using the unpaired Student’s t-test. Error bars denote mean ± S.D.; ns–not significant. Source data are provided as a Source Data file.
