Fig. 2: MS predominantly alters CSF cell composition and blood cell transcription.
From: Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis
a Comparative UMAP plots depicting only CSF cells from control (12,705 cells, left plot) and MS (9652 cells, right plot) donors. Color coding and cluster names are as in Fig. 1. b Volcano plot showing differences of cluster abundance of only CSF cells in MS samples compared to controls plotted as fold change (log10) against p value (−log10) based on beta-binomial regression. c Dotplot depicting selected genes differentially expressed in at least one cluster of MS cells compared to controls in CSF. Dot size encodes percentage of cells expressing the gene. Purple indicates higher, and turquoise indicates lower expression in MS, respectively. d Bayes factor (BF) frequency histogram in all cluster-specific case-control differential expression analyses colored by tissue. Higher magnification in bottom panel. Only clusters with a minimum of ten cells per tissue per disease state are included. Please note that the BF is proportional to the likelihood of differential expression (i.e., higher BF indicates more likely DE)28. Source data for (b, c) listing the differential expression values for MS vs. controls in CSF are provided in Supplementary Dataset 5. Source data for (d) listing the BFs for CSF vs. blood are provided in Supplementary Dataset 2.
