Fig. 1: Decidual ILC subsets identified by mass cytometry. | Nature Communications

Fig. 1: Decidual ILC subsets identified by mass cytometry.

From: Distinctive phenotypes and functions of innate lymphoid cells in human decidua during early pregnancy

Fig. 1

a Workflow summary for the analysis of cryopreserved matched blood and decidual samples by mass cytometry. b t-Distributed Stochastic neighbour embedding (tSNE) landscape of matched CD45+ Lineage negative (Lin−) (CD3, CD19, CD14, HLA-DR) decidual and peripheral blood mononuclear cells (n = 6). The algorithm incorporated markers indicated in final column of Supplementary Table 1 to generate the tSNE landscape. c Nearest-neighbour-based tissue probability plot indicates the relative distribution of decidual and peripheral blood derived CD45+ Lin− cells within an area of the tSNE landscape. Blue = surrounded by decidual cells, Red = surrounded by peripheral blood cells, White = surrounded by mixed cells. d The tSNE landscape is coloured according to relative expression intensity of indicated markers. Red = high expression, Blue = low expression. Cytofkit and R studio were used to perform these analyses. Source data are provided as a Source Data file.

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