Fig. 5: dNK granule content and organisation. | Nature Communications

Fig. 5: dNK granule content and organisation.

From: Distinctive phenotypes and functions of innate lymphoid cells in human decidua during early pregnancy

Fig. 5

a Granule content represented as geometric mean fluorescent intensity (gMFI) of side scatter in Lineage negative (Lin−) CD56+ cell subsets co-expressing 0–3 Killer-cell Immunoglobulin-like Receptors (KIRs, n = 11). Lines connect dNK subsets from the same individual. b Fold increase in side scatter over previous data-point in a. c Geometrical mean fluorescence intensity (gMFI) of Granzyme B in KIR2DS1-KIR2DS4- Lin-CD56+ cells co-expressing 0–2 inhibitory KIRs (n = 11). d Granzyme B gMFI in KIR2DL1-KIR2DL2/S2/3− Lin-CD56+ cells co-expressing 0–2 activating KIRs (n = 4). e Live Lin-CD56+CD94+dNK and pbNK were FACS-sorted and visualised using electron microscopy. Electron dense lysosomal structures are indicated with “L”. Data is representative of four donors. Scale bar indicates 1 μm. f Granule diameter measured by electron microscopy, aggregated data for multiple cells (n = 33 for dNK and 53 for pbNK). Red lines indicate mean. g Confocal microscopy pictures of pbNK (top) and dNK bottom) stained with the markers indicated (green), nucleus (blue) and cell outline (dashed line). Representative 2D views of multiple cells from five experiments are shown. White scale bar indicates 2 μm. h 3D representation of four pbNK (top) and two dNK cells (bottom) stained for centrosome (MTOC, pericentrin, red), CD107a/LAMP-1 (green) and nucleus (blue). Spots (CD107a/LAMP-1) and surfaces (pericentrin, DAPI for nucleus and Phalloidin for cell surface) created in Imaris are shown. The side of each square in the 3D cube represents 2 μm. i Quantification of multiple distance measurements from CD107a/LAMP-1 structure centre (green spots) to centrosome centre (red surfaces) in h. Line indicates mean. Dots represent average distances in each cell analysed (n = 18 and 9), from three different experiments with unique unmatched donors. a–c Stars indicate significance obtained with two-tailed one-way ANOVA of matched data points with Tukey correction. Tests were only performed on full series. **p < 0.01, ***p < 0.001, ****p < 0.0001. f, i Stars indicate significance obtained with a two-tailed Mann–Whitney test with 95% confidence level, ****p < 0.0001, **p < 0.001. Source data are provided as a Source Data file.

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