Fig. 5: Human LCs upregulate expression of IRF4 upon migration, but lack IRF8.
From: Genomic programming of IRF4-expressing human Langerhans cells
a IRF4, but not IRF8, protein expression is upregulated in BATF3-positive LC during migration from the epidermis. A representative FACS analysis of 3–5 independent donors, gates set using isotype controls for each antibody (nuclear staining for IRF4, IRF8 and BATF3). b IRF4 protein expression in steady-state vs migrated LCs. IRF4+ LCs (%) as measured by flow cytometry, median ± range, n = 4, three paired samples from independent donors, unpaired t test, box and whiskers show min and max value, line at median. Source data are provided as a Source Data file. c Transcript levels of key transcription factors in migrated LCs before and after TNF stimulation (2 h, 24 h). FPKM values, median ± range of three biological donors are shown. Source data are provided as a Source Data file. d Expression of IRF4 and BATF3 in response to TNF signalling (24 h). Representative graphs of five independent donors, gates set using isotype controls for each antibody (Nuclear staining for IRF4, IRF8 and BATF3).
