Fig. 2: Loss of Piezo1 in osteoblastic cells resulted in increased bone resorption.
From: Mechanical sensing protein PIEZO1 regulates bone homeostasis via osteoblast-osteoclast crosstalk
a, b FACS analysis of mSSCs, mBSCPs and mPre-BSCPs of bone marrow cells of 6-week-old male WT and Prx1Cre, Piezo1fl/fl mice. Data are mean ± SD, WT, n = 4, CKO, n = 6. c Representative images of Von kossa staining of 6-week-old male WT and Prx1Cre, Piezo1fl/fl mice. Scale bar = 500 μm d–g Histomorphometric analysis of proximal tibias from 6-week-old male WT and Prx1Cre, Piezo1fl/fl mice for bone volume per tissue volume (BV/TV) (d), trabecular number (Tb.N) (e), number of osteoblasts per bone perimeter (N.Ob/B.Pm) (f), osteoblast surface per bone surface (Ob.S/BS) (g). Data are mean ± SD, WT, n = 4, CKO, n = 3. h Representative images of dual Calcein-Alizarin red S labeling of proximal tibias from 6-week-old male WT and Prx1Cre, Piezo1fl/fl mice. Scale bar = 20 μm, n = 6. i–k Quantification of mineral apposition rate (MAR) (i), bone formation rate normalized to bone surface (BFR/BS) (j) and mineralizing surface normalized to bone surface (MS/BS) (k) of endo-cortical and trabecular bones of proximal tibias from 6-week-old male WT and Prx1Cre, Piezo1fl/fl mice. Data are mean ± SD, n = 6. l Serum PINP levels in 6-week-old male WT and Prx1Cre, Piezo1fl/fl mice. Data are mean ± SD, WT, n = 6, CKO, n = 8. m–o Histomorphometric analysis of the images from (c) for eroded surface per bone surface (ES/BS) (m), number of osteoclasts per bone perimeter (N.Oc/B.Pm) (n) and osteoclast surface per bone surface (Oc.S/BS) (o). Data are mean ± SD, WT, n = 4, CKO, n = 3. p Serum CTX-I levels in 6-week-old male WT and Prx1Cre, Piezo1fl/fl mice. Data are mean ± SD, WT, n = 6, CKO, n = 8. q Tartrate-resistant acid phosphatase (TRAP) staining of femurs isolated from 6-week-old male WT and Prx1Cre, Piezo1fl/fl mice. Scale bar = 50 μm. *P < 0.05; **P < 0.01. Two-tailed Student’s t test. Source data are provided in the Source Data File.
