Fig. 5: Col2α1 and Col9α2 mediated the inhibition of bone resorption by PIEZO1.
From: Mechanical sensing protein PIEZO1 regulates bone homeostasis via osteoblast-osteoclast crosstalk
a–d Osteoclastogenesis by OB-OC co-culture in vitro using BMSCs-derived osteoblasts from WT and Prx1Cre, Piezo1fl/fl mice. Scale bar = 100 μm. a TRAP staining of the osteoclasts in the co-culture system on the normal surface. b Co-culture supernatants were measured for TRAP activity via colorimetric readout (A405) on the normal surface. *P < 0.05; **P < 0.01. Two-tailed Student’s t test. Data are mean ± SD, n = 5. c TRAP staining of the osteoclasts (top panel) and resorption area (bottom panel) in the co-culture system on the bone biomimetic synthetic surface. d Quantification of the resorption area. *P < 0.05; **P < 0.01. Two-tailed Student’s t test. Data are mean ± SD, n = 6. e, f Indicated gene expression analysis of the cortical bones of WT and Prx1Cre, Piezo1fl/fl mice. *P < 0.05; **P < 0.01. Two-tailed Student’s t test. Data are mean ± SD, n = 4. (g, h) Immunofluorescence assay of COL2α1 (g) and COL9α2 (h) of the distal femurs of 3-day-old WT and Prx1Cre, Piezo1fl/fl mice. Scale bar = 100 μm. i, j QPCR analysis of Col2α1 (i) and Col9α2 (j) in the BMSCs-derived osteoblasts from WT and Prx1Cre, Piezo1fl/fl mice endured with 0.5 Hz, 1% intensity compression for 4 h by FlexCell compression system. *P < 0.05; **P < 0.01. Ordinary one-way ANOVA. Data are mean ± SD, n = 4. k–l Osteoclastogenesis by OB-OC co-culture in vitro using BMSCs-derived osteoblasts from WT and Prx1Cre, Piezo1fl/fl mice infected with Ctrl, Col2α1 and Col9α2 lenti-virus, respectively. k TRAP staining of the osteoclasts in the co-culture system. Scale bar = 100 μm. l Co-culture supernatants were measured for TRAP activity via colorimetric readout (A405). *P < 0.05; **P < 0.01. Ordinary one-way ANOVA. Data are mean ± SD, n = 3. Source data are provided in the Source Data File.
