Fig. 6: PIEZO1 promoted collagen expression through YAP nuclear localization.
From: Mechanical sensing protein PIEZO1 regulates bone homeostasis via osteoblast-osteoclast crosstalk
a Immunofluorescence assay of YAP in the cortical bones of WT and Prx1Cre, Piezo1fl/fl mice. Scale bar = 10 μm. b Quantification of YAP nuclear localization percentage in WT and Prx1Cre, Piezo1fl/fl mice. *P < 0.05. Two-tailed Student’s t test. Data are mean ± SD, n ≥ 5. c, d YAP expression by western blot (c) and indicated gene expression analysis (d) in the C3H10 cells infected with shControl and shYAP lenti-virus. e Indicated gene expression analysis in the C3H10 cells treated with DMSO or XMU-MP-1 (10 μm) for 24 h. f, g Luciferase assays showed that YAP could activate Col2α1 (f) and Col9α2 (g) promoters in the C3H10 cells. h, i Osteoclastogenesis by OB-OC co-culture in vitro using BMSCs-derived osteoblasts from WT and Prx1Cre, Piezo1fl/fl mice, the co-culture system was treated with DMSO or XMU-MP-1 (0.2 μm), respectively. h TRAP staining of the osteoclasts in the co-culture system. Scale bar = 100 μm. i Co-culture supernatants were measured for TRAP activity via colorimetric readout (A405). *P < 0.05; **P < 0.01. Ordinary one-way ANOVA. Data are mean ± SD, n = 4. Source data are provided in the Source Data File.
