Fig. 7: Sudden loss of Piezo1 in osteoblast lineage cells decreased the bone mass.

a Scheme of the experiment: male WT and Col1^{Cre ert2}, Piezo1^fl/fl mice were injected with tamoxifen (0.1 g/kg) at 8-week-old and sacrificed for analysis at 10-week-old. b 3D μ-CT images of trabecular bones of distal femurs. c–g μ-CT analysis of distal femurs for bone volume per tissue volume (BV/TV) (c), trabecular number (Tb.N) (d), trabecular thickness (Tb.Th) (e), trabecular spacing (Tb.Sp) (f) and cortical thickness (Ct.Th) of middle shaft of femurs (g). Data are mean ± SD, WT, n = 3, CKO, n = 4. h TRAP staining of the femurs isolated from 10-week-old male WT and Col1^{Cre ert2}, Piezo1^fl/fl mice. Scale bar = 50 μm. i Quantification of the TRAP staining by osteoclast surface per bone surface (Oc.S/BS). Data are mean ± SD, n = 3. j Immunofluorescence assay of COL2α1 of 10-week-old male WT and Col1^{Cre ert2}, Piezo1^fl/fl mice. Scale bar = 50 μm. *P < 0.05; **P < 0.01. Two-tailed Student’s t test. Source data are provided in the Source Data File.