Fig. 1: SFB+ microbiota is sufficient for cPTH to induce trabecular bone loss and stimulate bone turnover.
From: PTH induces bone loss via microbial-dependent expansion of intestinal TNF+ T cells and Th17 cells
SFB+ and SFB− mice from Taconic (TAC) and Jackson Laboratory (JAX) were treated with vehicle or cPTH for 2 weeks with antibiotics (Abx) or without antibiotics (No Abx). a The figure shows images of representative 3-dimensional μCT reconstructions of examined femurs. b Femoral trabecular bone volume fraction (BV/TV), n = 9–11 mice per group. c. Trabecular thickness (Tb.Th), n = 9–11 mice per group. d The images show representative tartrate resistant acid phosphatase (TRAP) stained sections of the distal femur. Original magnification Ă—40. Scale bar represents 300 μm. e Images are representative sections displaying the calcein double-fluorescence labeling. Original magnification Ă—20. Scale bar represents 300 μm f Number of osteoclasts per mm bone surface (N.Oc/BS), n = 8–10 mice per group. g Percentage of bone surface covered by osteoclasts (Oc.S/BS), n = 8–10 mice per group. h Mineral apposition rate (MAR), n = 8–10 mice per group. i Bone formation rate per mm bone surface (BFR/BS), n = 8–10 mice per group. j Serum levels of CTX, a marker of bone resorption, n = 8–10 mice per group. k Serum levels of osteocalcin, a marker of bone formation, n = 8–10 mice per group. All data are expressed as Mean ± SEM. All data were normally distributed according to the Shapiro-Wilk normality test. Data were analyzed by two-way ANOVA and post hoc tests applying the Bonferroni correction for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared to the indicated group. Source data are provided as a Source Data file.
