Fig. 2: SFB+ microbiota is sufficient for low calcium diet to induce trabecular bone loss and stimulate bone turnover. | Nature Communications

Fig. 2: SFB+ microbiota is sufficient for low calcium diet to induce trabecular bone loss and stimulate bone turnover.

From: PTH induces bone loss via microbial-dependent expansion of intestinal TNF+ T cells and Th17 cells

Fig. 2

SFB+ TAC and JAX mice and SFB- JAX mice were treated with control diet or low calcium diet for 4 weeks and with antibiotics (Abx) or without antibiotics (No Abx). a The figure shows images of representative three-dimensional μCT reconstructions of examined femurs. b Femoral trabecular bone volume/total volume fraction (BV/TV), n = 9–10 mice per group. c Trabecular thickness (Tb.Th), n = 9–10 mice per group. d Serum levels of CTX, a marker of bone resorption, n = 8–10 mice per group. e Serum levels of osteocalcin, a marker of bone formation, n = 9–10 mice per group. All data are expressed as Mean ± SEM. All data were normally distributed according to the Shapiro-Wilk normality test. Data were analyzed by two-way ANOVA and post hoc tests applying the Bonferroni correction for multiple comparisons. *p < 0.05, ***p < 0.001, and ****p < 0.0001 compared to the indicated group. Source data are provided as a Source Data file.

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