Fig. 3: SFB+ microbiota is sufficient for low calcium diet to alter bone structure and turnover in GF mice subjected to fecal material transfer. | Nature Communications

Fig. 3: SFB+ microbiota is sufficient for low calcium diet to alter bone structure and turnover in GF mice subjected to fecal material transfer.

From: PTH induces bone loss via microbial-dependent expansion of intestinal TNF+ T cells and Th17 cells

Fig. 3

Germ-free (GF) mice, SFB monoassociated GF mice, and GF mice colonized with JAX mice microbiota ±SFB were treated with control diet or low calcium diet for 4 weeks. a The figure shows images of representative three-dimensional μCT reconstructions of examined femurs. b Femoral trabecular bone volume/total volume fraction (BV/TV), n = 5–10 mice per group. c Trabecular thickness (Tb.Th), n = 5–10 mice per group. d Serum levels of CTX, a marker of bone resorption, n = 5–10 mice per group. e Serum levels of osteocalcin, a marker of bone formation, n = 5–10 mice per group. Data are expressed as Mean ± SEM. All data were normally distributed according to the Shapiro-Wilk normality test and analyzed by unpaired t-tests. *p < 0.05 and ***p < 0.001 compared to the indicated group. Source data are provided as a Source Data file.

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