Fig. 8: TNF is sufficient for cPTH and low calcium diet to upregulate CCL20 levels in the BM and recruit Th17 cells to the BM.

a Relative frequency of BM Vβ14 + Th17 cells in SFB⁺ TAC and JAX mice treated with cPTH or low calcium, diet with antibiotics (Abx) or without antibiotics (No Abx), n = 5–10 mice per group. b Relative frequency of BM Vβ14 + Th17 cells in GF mice, SFB monoassociated GF mice, and GF mice colonized with JAX mice microbiota ±SFB treated with control diet or low calcium diet, n = 5–10 mice per group. c, d Representative FACS plot and frequency of eGFP + Th17 cells in the BM of WT and Tnf−/− mice treated with Veh or cPTH and previously subjected to adoptive transfer of IL-17A-eGFP⁺ cells, n = 5 mice per group. e BM cells transcript levels of Ccl20 in SFB⁺ and SFB⁻ TAC and JAX mice treated with and without Abx and cPTH, n = 5 mice per group. f Transcript levels of Ccl20 in purified BM T cells, B cells, monocytes (Mon), dendritic cells (DCs) and stromal cells (SCs), n = 5 mice per group. g Frequency of PP and BM Th17 cells, and BM Vβ14 + Th17 cells in WT and Tnf−/− mice, n = 10 mice per group. h BM cells Ccl20 transcripts in WT and Tnf−/− mice, n = 5 mice per group. i BM cells Ccl20 transcripts in Tcrb−/− mice reconstituted with WT T cells or Tnf−/− T cells, n = 5 mice per group. j BV/TV, serum CTX levels and serum osteocalcin levels in WT and Tnf−/− mice, n = 10 mice per group. In panels F-I SFB⁺ JAX mice were treated with Veh or cPTH. Data are expressed as Mean ± SEM. All data were normally distributed according to the Shapiro-Wilk normality test. Data in a, d–j were analyzed by two-way analysis-of-variance and post hoc tests applying the Bonferroni correction for multiple comparisons. Data in panel b were analyzed by unpaired t-tests. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared to the indicated group. Source data are provided as a Source Data file.