Fig. 1: Comparison of methods for multiplexed ubiquitylome analysis.
From: Rapid and deep-scale ubiquitylation profiling for biology and translational research
a Conventional workflow for an anti-K-ɛ-GG antibody-based enrichment without isobaric labeling, where the antibody recognizes primary amine containing K-ɛ-GG epitope. b UbiFast workflow employing on-antibody labeling with isobaric mass tag reagents (e.g., TMT10/11) for anti-K-ɛ-GG antibody-based enrichment of ubiquitylated peptides.
