Fig. 1: TDP-43 loss induces lactation failure in mammary epithelium.
From: TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh
a Immunohistochemical (IHC) staining of TDP-43 in mammary glands of C57BL/6 female mice at different developmental stages (V, virgin; P17.5, pregnancy day 17.5; L1, lactation day 1; L2, lactation day 2; L10, lactation day 10; I3, involution day 3). Representative images (×40 objective magnification) from four mice are shown for each stage. Scale bar: 20 μm. b, c Pup survival rates from wild-type (WT) and Tardbp−/− female mice for first (b) and second (c) lactation, with seven pups per litter. WT represents Tardbpwt/wt+WAP-Cre mice. Tardbp−/− represents Tardbpfl/fl+WAP-Cre mice. n represents number of mothers. d Pup survival rates from WT and Tardbp−/− mothers after litter size was adjusted to seven for each foster mother on L2. e Body weights of surviving pups from WT and Tardbp−/− mothers. Pups nursed by their biological mother (WT or Tardbp−/−, black lines) and cross-nursed by their foster mother (Tardbp−/− or WT) at L2 (blue lines) and L10 (red lines) were recorded for each dam with seven pups beginning at L2. f Body weights of pups from second lactation after litter size was adjusted to seven on L2. Data are means ± SD. Source data are provided as a Source Data file.
