Fig. 3: Larger lipid droplets are present in MECs after knockout of Tardbp.
From: TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh
a, b Phase contrast micrographs (a) and statistics (b) of milk fat globules (MFGs) from wild-type (WT) and Tardbp−/− mice analyzed using Image-Pro Plus 5.0 at lactation day 2 (L2) (left, n = 7 mice) or L10 (right, n = 6 mice). Scale bar: 100 μm. Data are means ± SD. Unpaired t test was used to evaluate statistical significance. ***P < 0.001. c Hematoxylin and eosin staining of mammary glands from WT and Tardbp−/− mice at L1, L2, and L10. Magnified areas at L10 are shown in red boxes (left). Arrowhead shows cytoplasmic lipid droplets (CLDs) in cells and arrow shows large lipid droplets (LDs) in alveolar lumen. Scale bar: 20 μm. d Immunofluorescence staining of adipophilin (PLIN2) (red) showing LDs in alveoli of mammary glands from WT and Tardbp−/− mice at L1 and L2. Sections were also stained with wheat germ agglutinin (WGA) (green) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) to outline luminal border or nuclei. Magnified areas (right) are shown in white boxes (left). Scale bar: 10 μm. e Electron microscopy images of mammary gland sections from WT and Tardbp−/− mice at L1. Arrowhead indicates nucleus and asterisk shows lumen of mammary glands. Scale bar: 2 μm. Source data are provided as a Source Data file.
