Fig. 4: TET3 induces epigenetic modification at the P2 promoter.

a Left, DMR sequence in the P2 promoter of mouse Hnf4α. The three differentially methylated CpGs are highlighted. Numbers mark the starting and ending positions of nucleotides in the chromosome. Right, schematic of mouse Hnf4α showing TET3/FOXA2 ChIP and hMeDIP (red) and RNAP ChIP (green) regions, respectively. Numbers mark the starting and ending positions of nucleotides in the chromosome. Not drawn to scale. b Mouse primary hepatocytes were treated with vehicle (−) or 20 nM of glucagon (+) for 24 h, followed by ChIP-qPCR of P2 and P1 promoters. Data are presented as % input, with gray bars representing background IgG signal. n = 3. c Left, mouse hepatocytes were treated as in b, followed by hMeDIP of P2 and P1 promoters. n = 3. Right, mouse hepatocytes were infected with Ad-GFP or Ad-TET3, followed by hMeDIP at 36 h post-infection. n = 3. d Left, mouse hepatocytes were treated as in b, followed by QMSP of P2. Right, H19 KO hepatocytes were infected with Ad-GFP or Ad-TET3, followed by QMSP of P2 at 48 h post-infection. n = 3. e Left, mouse hepatocytes were treated with vehicle or glucagon for 24 h, followed by RNAP ChIP of P2 and P1 promoters. Right, H19 KO mouse hepatocytes were infected with Ad-GFP or Ad-TET3, followed by RNAP ChIP at 48 h post-infection. n = 3. Data b–e are representative of two independent experiments and are presented as mean ± SEM, using Two-tailed Student’s t tests. *p < 0.05, **p < 0.01.