Fig. 3: Effects of nctA and nctB deletion on the global and the ergosterol biosynthetic gene expression.

a, b Comparison of differential gene expression in the nctA and the nctB null mutants. Scatter plot comparison of the log2 differential expression ratio of gene expression data in (a) no-drug, and (b) 0.5 mg/L itraconazole conditions. The log2 expression ratio between the nctA null mutant and the wild-type are plotted on the x-axis, and the nctB null mutant and the wild-type are plotted on the y-axis. The Pearson’s correlation (r) between the two gene expression data sets is shown. c, d Effects of nctA deletion on the expression of the genes involved in ergosterol biosynthesis. c The putative ergosterol biosynthetic pathway in A. fumigatus. The genes highlighted in yellow are those whose expression levels were upregulated more than 1.5-fold in the nctA null mutant compared with the wild-type. The genes highlighted in light blue are those whose expression levels were downregulated more than 1.5-fold in the nctA null mutant compared with the wild-type. b Heatmap showing the RNA-seq expression levels of the genes involved in ergosterol biosynthesis. Log2 differential expression values are scaled between −1.5 and 1.5, and displayed. Source data are provided as a Source Data file.