Fig. 5: Loss of nctA and nctB increases the cellular ergosterol content.
From: The negative cofactor 2 complex is a key regulator of drug resistance in Aspergillus fumigatus
Ergosterol levels of the wild-type (WT), the nctA null mutant (∆nctA), the nctB null mutant (∆nctB) and the nctA reconstituted isolate (nctA rec) in RPMI-1640 incubated for 24 h were determined by GC–MS. Ergosterol content of each mutant was normalised to that of the wild-type, and shown as a relative fold change. Samples were assessed in biological triplicates. P-values were calculated using one-way ANOVA: *P < 0.05; ns, P > 0.05. The error bars represent standard error of the mean. Source data are provided as a Source Data file.
