Fig. 8: In vivo PDT efficiency assessments.
From: Self-assembled single-atom nanozyme for enhanced photodynamic therapy treatment of tumor
a Relative tumor volumes of mice after various treatments (control, Ce6, MCC, and OxgeMCC-r, n = 5). The last three groups were treated with laser irradiation: 5 min with a power density of 100 mW cm−2. Injection dose is 100 µL, with a Ce6 concentration of 4 mg kg−1. b Photographic images of tumors excised from different groups after various treatments indicated. c Average weights of tumors from different groups of mice after various treatments indicated. d Representative hypoxia immunofluorescence images of tumor slices. Nuclei, blood vessels, and hypoxic regions were stained by 4’,6-diamidino-2-phenylindole (DAPI, blue), anti-CD31 antibody (red), and anti-pimonidazole antibody (green), respectively. Scale bar is 100 µm. e H&E staining and f Ki-67 staining of tumor slices from different groups after various treatments. Scale bar is 50 µm. g TUNEL staining of tumor slices from different groups indicated. Scale bar is 50 µm. h MR imaging of tumor-bearing mouse at different treatment points. Data are presented as mean ± s.e.m. (n = 5). Statistical analysis was performed via one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001.
