Fig. 3: cf-mRNA reflects transcriptional activity of hematopoietic lineages during BM reconstitution. | Nature Communications

Fig. 3: cf-mRNA reflects transcriptional activity of hematopoietic lineages during BM reconstitution.

From: Non-invasive characterization of human bone marrow stimulation and reconstitution by cell-free messenger RNA sequencing

Fig. 3

a, b Heat map of time-varying transcripts identified by cf-mRNA-Seq on multiple myeloma (MM) (a) and acute myeloid leukemia (AML) (b) patients undergoing BM ablation, followed by autologous or allogenic stem cell transplant, respectively (at day 0). Each column represents a time point with respect to the time of transplant, indicated in the bottom. Each row represents a gene. Enriched gene ontology terms for each cluster of transcripts are indicated (adjusted p value). c–h Time course of the levels of erythrocyte (red, c, d), megakaryocyte (green, e, f) and neutrophil (gray, g, h) specific transcripts in indicated MM (c, e, g) and AML (d, e, h) patients throughout the study. (Transcript identity is provided in Supplementary Table 5, detectable genes <5000 TPM used for visualization). Corresponding peripheral blood counts (every 3 days) are plotted in the secondary axis and represented with a black dotted line (RBC count, millions per mcL (c, d), platelet count, thousands per mcL (e, f) and neutrophil count, thousands per mcL (g, h). Day of blood collection with respect to transplant is indicated in the X axis. i, j Relative variation of progenitor neutrophil transcripts in AML patients 1 (i) and 2 (j) throughout the study. Average percent change for these transcripts is represented with a dashed blue lane. Dashed black line shows neutrophil counts in blood.

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