Fig. 3: Functional analysis of BOC1.
From: A common wild rice-derived BOC1 allele reduces callus browning in indica rice transformation
a–d Phenotypes of calli from pRi-BOC1-YIL25 transgenic plants (n = 18). Negative represents segregating plants from the transformation process but without the transgene. Scale bars, 1 cm. e Comparison of the relative expression levels and CBI between a negative transgenic line and 10 RNAi lines (n = 3 biological replicates). f–i Phenotypes of calli from pCPL-BOC1-Teqing transgenic plants (n = 15). Scale bars, 1 cm. j Comparison of the relative expression levels and CBI in a negative transgenic line and 10 complementation lines (n = 3 biological replicates). k–n Phenotypes of calli from pOE-BOC1-Teqing transgenic plants (n = 22). o Comparison of the relative expression levels and CBI in a negative transgenic line and 10 overexpression lines (n = 3 biological replicates). The black line indicates the trend of the relative expression levels, and the gray line indicates the trend of the CBI. Scale bars, 1 cm. Data are means ± SE, two-tailed Student’s t-tests. Source data underlying Figs. e, j, o are provided as a Source Data file.
