Fig. 2: Extracellular arginine is essential for RANKL-induced osteoclastogenesis.
From: Environmental arginine controls multinuclear giant cell metabolism and formation
a Schematic of systems-wide approaches performed to understand the effect of recArg1 on RANKL dependent osteoclastogenesis. b KEGG metabolic enrichment of differentially expressed genes upon RANKL treatment. c Representative TRAP stainings depicting that recArg1 abolishes murine and human RANKL-induced osteoclastogenesis. d Quantification of murine data depicted in c (n = 6). e qRT-PCR time course of Nfatc1 and Fos (n = 3). f Quantification of viable cells 24 h post RANKL/recArg1 treatment (n = 4). g, h Osteoclastogenesis is unchanged by Arg1 deficiency. TRAP stainings (g) and Western blots of ARG1 in preosteoclasts (Pre-OC) and osteoclasts (OC) (h). i qRT-PCR time course of Arg1 (n = 3). Data are mean ± SEM, *P < 0.05, ***P < 0.001, ****P < 0.0001, one-way ANOVA (d) and two-way ANOVA post-hoc pairwise comparisons with Bonferroni correction (e). Scale bar represents 200 µm (c, g). Source data are provided as a Source Data file.
