Fig. 2: Depletion of AKAP8 promotes an EMT phenotype. | Nature Communications

Fig. 2: Depletion of AKAP8 promotes an EMT phenotype.

From: The RNA-binding protein AKAP8 suppresses tumor metastasis by antagonizing EMT-associated alternative splicing

Fig. 2

a Western blot analysis of EMT markers using lysates from HMLE/Twist-ER cells expressing control shRNA (Ctrl) or AKAP8 shRNAs (KD-1, KD-2). Lysates were collected before (untreated) and after 12 days of tamoxifen (TAM) induction. b Phase-contrast images (× 10) displaying cell morphology differences between control (Ctrl) and AKAP8-silenced HMLE/Twist-ER cells before (untreated) and after 12 days of TAM induction. White line represents scale bar at 100 μm. c Immunofluorescence images (× 40) showing the loss of E-cadherin at cell junction 12 days after TAM treatment in the AKAP8 knockdown cells. Green: E-cadherin, Blue: DAPI. d Phase-contrast images of HCT116 cells showing cell morphology changes upon AKAP8 knockdown. White line represents scale bar at 100  μm. e Western blot analysis of the epithelial markers in HCT116 cells expressing control or AKAP8 shRNA. Source data are provided as a Source Data file.

Back to article page