Fig. 6: TP53−/−;PDGFRAΔ8–9 iHGG shows prominent karyotype abnormalities accompanied by extrachromosomal DNA. | Nature Communications

Fig. 6: TP53−/−;PDGFRAΔ8–9 iHGG shows prominent karyotype abnormalities accompanied by extrachromosomal DNA.

From: Longitudinal assessment of tumor development using cancer avatars derived from genetically engineered pluripotent stem cells

Fig. 6

a DAPI staining of PTEN−/−;NF1−/− primary iHGG cells, scale bar, 10 μm. b DAPI staining of TP53−/−;PDGFRAΔ8–9 primary iHGG cells. Red arrows indicate ecDNA, scale bars, 10 μm (left), 2 μm (right). c DAPI staining of TP53−/−;PDGFRAΔ8–9 secondary iHGG cells. Red arrows indicate ecDNA, scale bars, 10 μm (left), 2 μm (right). d EdU labeling of chromosomes and ecDNA in a metaphase spread of TP53−/−;PDGFRAΔ8–9 secondary iGBM, scale bar, 5 μm. e Spectral karyotyping analysis of TP53−/−;PDGFRAΔ8–9 iHGG cells.

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