Fig. 5: PIO restores IFN-γ production in human tumor-infiltrating iNKT cells in vitro.
From: Impaired lipid biosynthesis hinders anti-tumor efficacy of intratumoral iNKT cells
a–c PPARγ (a), SREBP1 (b), and Fillipin III staining (c) in tumor-infiltrating (TI) and para-carcinoma (PC) iNKT cells from HCC patients. d, e Correlations between PPARγ and SREBP1 (d), PPARγ and Fillipin III (e), in TI and PC iNKT cells as described in a. f Intracellular IFN-γ staining (left), percentages of IFN-γ+ iNKT cells (middle), and mean fluorescence intensity (MFI) of anti-IFN-γ (right) in TI and PC iNKT cells from HCC patients. g Influences of water-soluble cholesterol on percentages of IFN-γ+ iNKT cells and MFI of anti-IFN-γ in TI iNKT cells from HCC patients. h–j Influences of PIO on SREBP1 expression (h), Filipin III staining (i), and percentages of IFN-γ+ iNKT cells and MFI of anti-IFN-γ (j) in TI iNKT cells from HCC patients. Data are means ± SEM of 13 patients (a–c), 6 patients (f, h), 5 patients (g, i), or 8 patients (j). Data were analyzed by paired student’s t-test (a–c), Mann–Whitney test (f–j), or Pearson’s correlation test (d, e). *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file.
