Fig. 1: Design principles.
From: Decorating bacteria with self-assembled synthetic receptors
a One way to decorate E. coli with artificial receptors, which are appended with a specific functionality (X), involves the binding of X-ODN-1 to a hexa-histidine tag (His-tag) fused to OmpC (I → II). This process can be reversed by subjecting the bacteria to EDTA (II → I). Another way to introduce an unnatural recognition motif (Y) to the bacterial surface is by adding to the bacteria decorated with ODN-1 a complementary strand modified with the desired functionality (Y-ODN-2, II → III). Y-ODN-2 can be selectively removed by adding a complementary strand, ODN-3 (III → II). b Structure of X-ODN-1.
