Fig. 4: Programing the bacteria to interact with proteins and cancer cells.
From: Decorating bacteria with self-assembled synthetic receptors
a Schematic illustration of an experiment in which modified His-tagged bacteria were treated with Alexa 647-modified streptavidin (Alexa-SA). Left: Bacteria modified with a D1 duplex generated from ODN-1 and biotin-ODN-2. Right: Bacteria modified with a D0’ duplex that lacks biotin. b The resulting images (merged bright-field and fluorescence). c Images recorded following the incubation of the bacteria bound to Alexa-SA with ODN-3. d Schematic illustration of an experiment in which modified His-tagged bacteria were incubated with KB-cells. Left: Bacteria decorated with a D2 duplex consisting of ODN-1 and TAMRA-labeled folate-ODN-2. Right: Bacteria decorated with a D0 duplex that lacks the folate group. e The resulting images. f Images obtained after treating the bacteria that are bound to KB cells with ODN-3. g Incubating KB-cells with the D2 duplex (in the absence of bacteria) did not lead to fluorescence labeling of the KB-cell. h Representative flow cytometry histograms of KB cells before (gray) and after (yellow/cyan) treatment with: (left) D2-modified bacteria (16 × 108 cells/ml), (middle) D2 alone or D0-modified bacteria (500 nM), and (right) anti-FR antibody (0.31 μg/ml). i Bright field and fluorescent images of healthy MCF-10A cells (left) and cancerous KB cells (right) treated with the D2-modified bacteria.
