Fig. 2: WM4196 minicells are suitable for high-resolution analysis of chemoreceptor arrays.
From: Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
a Representative electron micrographs showing healthy looking WM4196 minicells. b—left XY slice and b—right YZ slices through tomograms of WM4196 minicells showing that they appear flattened in vitreous ice, yielding thin samples suitable for cryo-ET. Black dotted lines are used to show membrane positions which are not clear in the images due to missing-wedge effects from tomographic reconstruction. c slices through tomograms of WM4196 minicells exhibiting presence of chemoreceptor arrays aligned both perpendicular (left) and parallel (right) to the electron beam. Scale bar is 100 nm in all panels.
