Fig. 3: Extreme instability of ER in AD model mice revealed by in vivo ER imaging. | Nature Communications

Fig. 3: Extreme instability of ER in AD model mice revealed by in vivo ER imaging.

From: YAP-dependent necrosis occurs in early stages of Alzheimer’s disease and regulates mouse model pathology

Fig. 3

a In vivo ER and Aβ images were acquired by two-photon microscopy from 1-month-old 5xFAD mice into which ER-tracker and BTA1 had been injected in one shot 4 h before observation. ER and Aβ image sets were taken in tandem every 20 min. 3D images of ER and Aβ stains were merged by IMARIS (Bitplane, Zurich, Switzerland). Dot-line indicates a single neuron. b Total volumes of ER puncta belonging to a single cell were quantified by IMARIS (Bitplane, Zurich, Switzerland), and time courses are shown in the graph. Changes were more pronounced in 5xFAD mice than in non-transgenic sibling mice (Non-Tg sibling). N = 3 mice, n = 9 cells. c To verify the finding in b, standard deviation (SD) and quartile deviation of ER volumes from a single cell at multiple time points were compared between groups of 5xFAD and non-transgenic sibling mice. Box plots show the median, quartiles and whiskers that represent 1.5× the interquartile range. P-values were determined by Welch’s test, **p < 0.01 (N = 3 mice, n = 9 cells). Source data are provided as a “Source Data file”.

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