Fig. 4: Glutaminase 1 is highly expressed in UPS.

a Gls mRNA levels in C2C12, C2C12 D3, C2C12 D6, KP-6634, and KPH2-7215 cells. N = 3 with three technical replicates. Data represent mean ± s.e.m.; *p < 0.05, **p < 0.005. b Gls mRNA levels in gastrocnemius muscle, KP, and KPH2 tumours. N = 5 with three technical replicates. Data represent mean ± s.e.m. *p < 0.05. c Representative GLS staining in KP (left; n = 8) and KPH2 (right; n = 8) tumours. Asterisks indicate unstained skeletal muscle. Scale bars = 100 μm. d GLS staining of human skeletal muscle (left; N = 5) and undifferentiated pleomorphic sarcoma (UPS; right; N = 11). Scale bars = 100 μm. e Quantification of GLS staining intensity in an STS microarray. f GLS mRNA expression from Oncomine analysis of Barretina et al.⁴³ (left) and Detwiller et al.⁴⁴ (right) sarcoma patient samples datasets. Values are normalized to median-centred intensity and shown on a log₂ scale. Dediff. lipo., dedifferentiated liposarcoma; pleo. lipo., pleomorphic liposarcoma; UPS, undifferentiated pleomorphic sarcoma; *p < 0.05. g GLS mRNA expression from Oncomine analysis of Gibault et al.⁴⁵ leiomyosarcoma patient samples datasets. Values are normalized to median-centred intensity and shown on a log₂ scale. PD, poorly differentiated; WD, well differentiated. p-Values were calculated from a two-tailed Student’s t-test for a, b, f, g. Source data are provided as a Source Data file.