Fig. 5: Glutaminase inhibition with CB-839 and effects on UPS cells.
From: Targeting glutamine metabolism slows soft tissue sarcoma growth
a Proliferation of C2C12s, KP-6634s, and KPH2-7215s (left to right) grown in media with or without glutamine (Q) and treated with 1 μM CB-839. N = 3 with three technical replicates. Data represent mean ± s.e.m.; *p < 0.05, ***p < 0.0005. b Viability of C2C12, C2C12 D3, C2C12 D6, KP-6634, and KPH2-7215 cells grown in media with or without Q, treated with 1 μM CB-839 and assessed after 48 h. N = 3 with two technical replicates. Data represent mean ± s.e.m.; *p < 0.05, **p < 0.005, ***p < 0.0005. c Proliferation of C2C12s, KP-6634s, and KPH2-7215s (left to right) grown in media with or without Q, treated with 1 μM CB-839, and 5 mM dimethyl 2-oxoglutarate (DKG) or 2 mM glutamate (E). N = 3 with three technical replicates. Data represent mean ± s.e.m.; ***p < 0.0005. d Viability of C2C12, C2C12 D3, C2C12 D6, KP-6634, and KPH2-7215 cells grown in media with or without Q, treated with 1 μM CB-839, 5 mM DKG, or 2 mM E, and assessed after 48 h. N = 3 with two technical replicates. Data represent mean ± s.e.m.; ***p < 0.0005. p-Values were calculated from a two-tailed Student’s t-test for a–d. Source data are provided as a Source Data file.
