Fig. 1: JMJD3 epigenetically activates hepatic autophagy-network genes.

JMJD3-floxed mice were infected with AAV-TBG-Cre or control AAV-GFP for 12 weeks, and then, fasted for 16 h. a Experimental outline (top) and the levels of JMJD3 detected by IB in the liver and intestine (bottom). b RNA-seq: heat maps of changes in mRNA levels of autophagy-related genes (n = 3 mice). c ChIP-seq: normalized H3K27-me3 peaks at hepatic genes involved in autophagic pathways (UCSC genome browser). d Venn diagram (top) of downregulated genes and genes with increased H3K27-me3 levels after liver-specific downregulation of JMJD3 and G/O analysis (bottom) of the hepatic genes that show both increased H3K27-me3 levels and decreased expression. e The mRNA levels of the indicated genes in liver of mice fasted for 16 h (Fs) or refed (Fd) for 6 h after fasted for 10 h were measured by q-RTPCR (n = 5–10 mice). f, g ChIP assays: effects of the downregulation of JMJD3 on f H3K27-me3 levels and g occupancy of JMJD3 at the indicated genes (n = 3 mice). Source data are provided as a Source Data file. All values are presented as mean ± SD. Statistical significance was measured using the e–g two-way ANOVA with the Bonferroni post-test. *P < 0.05, **P < 0.01, and NS statistically not significant.