Fig. 8: Expression of KLB in obese mice restores FGF21 signaling and autophagy.

a Mice that had been fed a ND or HFD for 12 weeks were treated i.v. for 3 h with 0.1 mg/kg FGF21, and hepatic levels of the indicated proteins were detected by IB (left, n = 3 mice). The ratios of band intensities for the indicated proteins are shown (right). b Mice were fed a HFD for the times indicated and mRNA levels of Klb, JMJD3, Fgfr1, and Fgfr4 were measured by q-RTPCR (n = 3 mice). c Mice fed a HFD for 8 weeks were injected with AAV-TBG-KLB or AAV-TBG-GFP. Four weeks later, the mice were treated i.v. with vehicle or 0.1 mg/kg FGF21 for 3 h, and hepatic protein levels of the indicated proteins were detected by IB (n = 2 mice). Source data are provided as a Source Data file. a, b Values are presented as mean ± SD. Statistical significance was measured using the a two-way ANOVA with the Bonferroni or b one-way ANOVA with the Dunnett post-test *P < 0.05, **P < 0.01, and NS, statistically not significant.