Fig. 7: LInDA tools affect epithelial integrity in vivo. | Nature Communications

Fig. 7: LInDA tools affect epithelial integrity in vivo.

From: An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells

Fig. 7

a Diagram of the experimental setup. Xenopus embryos were injected with DHFR-cyto and E-cadherin-Δcyto-Halo at the two-cell stage and imaged at stage 10.5, with or without the dimerizer and with or without exposure to a 405 nm laser. b–i Epidermal dissociation was observed by co-injection of DHFR-cyto and E-cadherin-Δcyto-Halo (d, e) compared to wild type controls (b, c), which was rescued by incubation with the dimerizer (f, g). Dissociation was induced by photocleavage under a 405 nm laser (h, i). j–u, DHFR-cyto is cytosolic and E-cadherin-Δcyto-Halo is localized to the cell contact (j–l). Upon the addition of Ha-pl-TMP dimerizer, DFHR-cyto translocates to the cell contact (m–o), which can be disrupted by exposure to blue light (p–r). Blue light fails to prevent accumulation of DHFR-cyto at the cell contact when embryos are incubated with the non-photocleavable Ha-TMP dimerizer (s–u).

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