Fig. 4: E–M coupling interactions when the VSD adopts the activated conformation.
From: Two-stage electro–mechanical coupling of a KV channel in voltage-dependent activation
a, b Double mutant cycle analysis of the interaction between L251 and I268. ∆∆G = ∆G – (∆G1 + ∆G2) = 1.8 kcal/mol, which indicates interaction between the two residues. n ≥ 3. All averaged data are shown in mean ± SEM. c Double mutant cycle analysis of interactions between W248 and I268, L251 and I268, L251 and F339, M238 and L271, and L239 and L271 (∆∆G = 1.4–4.4 kcal/mol). n ≥ 3. d Mapping the five pairs of interacting residues onto the KV7.1 cryoEM structure. Color codes are the same as in Fig. 3h. Source data are provided as a Source Data file.
