Fig. 3: Nano-sapper reversed CAFs activation and reduced stromal composition in PDAC. | Nature Communications

Fig. 3: Nano-sapper reversed CAFs activation and reduced stromal composition in PDAC.

From: Dual-mechanism based CTLs infiltration enhancement initiated by Nano-sapper potentiates immunotherapy against immune-excluded tumors

Fig. 3

a Six-week-old male C57BL/6 mice were orthotopically inoculated with KPC1199 cells (1 × 106) on day 0. Saline, FHK-CaMP (MP = 13.9 mg/kg), FHK-pLIGHT@CaP (25 μg plasmid per mouse) and Nano-sapper (MP = 13.9 mg/kg, 25 μg plasmid per mouse) were i.v. administered on day 11, 13, and 15. On day 16, tumors were harvested for analysis. b–d Tumor slices were stained for α-SMA, FAP, fibronectin and collagen, and analyzed by ImageJ. Scale bars, 100 μm (n = 10 section images from three mice). Data were pooled from three independent experiments. e, f Tumors were lysed to detect expression of α-SMA, FAP and fibronectin (n = 3 mice). Data were pooled from three independent experiments. Data are presented as mean ± s.d. One-way ANOVA with Bonferroni multiple comparisons post-test was used for (c), (d), and (f). Error bars represent s.d. Source data of (e) are provided as a Source Data file.

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