Fig. 2: The energy stress pathway mediates a σB expression gradient from the top of the biofilm. | Nature Communications

Fig. 2: The energy stress pathway mediates a σB expression gradient from the top of the biofilm.

From: Stochastic pulsing of gene expression enables the generation of spatial patterns in Bacillus subtilis biofilms

Fig. 2

a–c Confocal microscopy images of slices of central parts of 48 h biofilm colony with merged channels for constitutive fluorescent reporter, PsigA-RFP, in red and PsigB-YFP in green. a WT cells, b ΔrsbRU cells, c ΔrsbQP cells. Scale bar 100 μm. d The ratio of PsigB-YFP to PsigA-RFP expression levels in the three strains as measured from the top of the colony, error bars indicate the SEM (WT: n = 13 images from three experiments, ΔrsbRU: n = 10 from two experiments, ΔrsbQP: n = 11 from two experiments). Source data are provided as a Source Data file.

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