Fig. 2: MTR4 is required for glycolysis of HCC cells.
From: MTR4 drives liver tumorigenesis by promoting cancer metabolic switch through alternative splicing
a Global gene expression analysis of iMTR4 cells before and after Doxy treatment identified differential expressed genes (DEGs), which were enriched in various pathways with pathway analysis and visualized as the overlap graph. b The mRNA levels of glycolytic genes in iSC and iMTR4 cells before and after Doxy treatment. Two-tailed, paired t-test. Data are presented as mean value ± s.d. p value is indicated. n.s., non-significant. n = 3 biologically independent samples. c, d Extracellular acidification rate (ECAR) in control cells and MTR4 KD cells with either MMC (5 µg/ml) or DMSO as a solvent control in response to glucose (10 mM), oligomycin (1 μM), and 2-DG (50 mM). n = 3 independent experiments. Data are presented as mean value ± s.d., two-way ANOVA with a Tukey’s multiple comparison test. p value is indicated. e, f Oxygen consumption rate (OCR) in control cells and MTR4 KD cells with either MMC or DMSO as a solvent control in response to oligomycin (1 μM), fluorocarbonyl cyanide phenylhydrazone (FCCP, 1.5 μM), rotenone/antimycin A (Rot/AA, 0.5 μM). n = 3 independent experiments for each group. Data are presented as mean value ± s.d., two-way ANOVA with Tukey’s multiple comparison test. p value is indicated. Res., respiration; Cap, capability. Source data are provided as a Source Data file.
