Fig. 1: Glu-CB1-KO mice display resilience to food addiction.

a Timeline of the experimental sequence of the food addiction mouse model. b Reduced number of reinforcers during 1 h of operant training sessions maintained by chocolate-flavored pellets in Glu-CB1-KO compared to WT mice (mean ± S.E.M, repeated measures ANOVA, genotype effect ***P < 0.001). c–e Glu-CB1-KO mice decreased response in the three addiction-like criteria tests (individual data with median and interquartile range, U Mann–Whitney, **P < 0.01): c Persistence to response. d Motivation. e Compulsivity. The dashed horizontal line indicated the 75th percentile of distribution of WT mice, it is used as the threshold to consider a mouse positive for one criterion. Addicted mice in gray filled circles for WT and blue for Glu-CB1-KO mice. f Reduced percentage of mice categorized as addicted in Glu-CB1-KO compared to WT mice at the late period (chi-square, **P < 0.01). g–i Pearson correlations between individual values of addiction-like criteria and g non-reinforced active responses in 10 min, h breaking point in 5 h and i number of shocks in 50 min (n = 56 for WT mice and n = 58 for Glu-CB1-KO mice; see also Supplementary Figs. 1 and 2; statistical details are included in Supplementary Table 1).