Fig. 1: Fibrinogen deposition in the SVZ after cortical injury. | Nature Communications

Fig. 1: Fibrinogen deposition in the SVZ after cortical injury.

From: Fibrinogen induces neural stem cell differentiation into astrocytes in the subventricular zone via BMP signaling

Fig. 1

a GFAP immunostaining (green) around the lesion core (indicated by yellow dotted line) and in the SVZ. White box indicating enlargement of SVZ showing GFAP expression in the adult SVZ 7 days after PT (right) (n = 4 mice). Scale bars, 369 μm, left and 57 µm, enlargement. b Fibrinogen immunostaining (red) in the lesion core and in the SVZ 1 day after PT. White box indicating enlargement of SVZ showing fibrinogen deposition in the SVZ 1 day after PT (right) (n = 4 mice). Scale bars, 428 μm, left and 120 µm, enlargement. c GFAP (green) and fibrinogen (red) immunostainings in the lesion core (left) and in the SVZ (right) 3, 5 and 7 days after stroke (MCAO). White box indicating enlargement of SVZ showing increased GFAP expression colocalizing with fibrinogen (red) deposition in the SVZ 3 days after stroke. Scale bars, 543 μm, left and 50 µm, right (n = 3 mice). d δ-GFAP (green) and fibrinogen (red) immunostainings in the SVZ of patients after stroke compared to healthy controls. White box indicating quantification area. Scale bar, 25 μm. Quantification of fibrinogen immunoreactivity in the SVZ per area (n = 5 (healthy control), n = 6 (stroke patients, mean ± s.e.m., unpaired Student’s t-test, *P < 0.05). e Representative images of S100β + ependymal cells (green) lining the lateral ventricle and adjacent fibrinogen (red) in the SVZ 5 h after PT (PT Day 0). Scale bar, 40 μm. f CD31 + endothelial cells (green) and fibrinogen (red) in the SVZ and striatum 5 h after PT (PT Day 0). White boxes indicating enlargement of fibrinogen deposition around blood vessels in SVZ (right, bottom) compared to no fibrinogen deposition around blood vessels in the striatum (right, top) after PT. Scale bars, 40 μm, left and 95 µm, enlargement. g GFAP + astrocytes (green) and fibrinogen (red) in the ipsilateral SVZ at different days after PT and uninjured mice. Scale bar, 57 μm. Quantification of GFAP + cells and fibrinogen immunoreactivity in the SVZ per area (n = 3 mice, mean ± s.e.m., one-way ANOVA and Bonferroni’s multiple comparisons test, *P < 0.05, ****P < 0.0001). SVZ subventricular zone.

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